mouse anti-nucleolin antibody  (Wanleibio)

Journal: Frontiers in Microbiology

Article Title: Anaplasma phagocytophilum AFAP targets the host nucleolus and inhibits induced apoptosis

doi: 10.3389/fmicb.2024.1533640

Figure Lengend Snippet: Top 25 most abundant AFAP-interacting proteins.

Article Snippet: Permeabilized cells were incubated with a mouse monoclonal anti-nucleolin antibody (cat#:sc-17826, Santa Cruz Biotechnology) or mouse monoclonal anti-NPM1 antibody (cat#: sc-32256, Santa Cruz Biotechnology) at the dilution of 1:100 for 1 h at 37°C before incubation with Alexa Fluor 555-conjugated goat anti-mouse IgG antibody (1:200 dilution, Molecular Probes, Invitrogen).

Techniques:

Journal: Frontiers in Microbiology

Article Title: Anaplasma phagocytophilum AFAP targets the host nucleolus and inhibits induced apoptosis

doi: 10.3389/fmicb.2024.1533640

Figure Lengend Snippet: Colocalization assay for AFAP and nucleolin, and AFAP and NPM1. RF/6A cells were transfected for 1 day with a plasmid expressing AFAP-GFP (A,B) or GFP (C,D) , followed by incubation with mouse monoclonal anti-nucleolin (A,C) or anti-nucleophosmin (B,D) antibody and Alexa Fluor 555-conjugated goat anti-mouse IgG antibody. DAPI was used to stain the nucleus. Scale bars: 10 μm.

Article Snippet: Permeabilized cells were incubated with a mouse monoclonal anti-nucleolin antibody (cat#:sc-17826, Santa Cruz Biotechnology) or mouse monoclonal anti-NPM1 antibody (cat#: sc-32256, Santa Cruz Biotechnology) at the dilution of 1:100 for 1 h at 37°C before incubation with Alexa Fluor 555-conjugated goat anti-mouse IgG antibody (1:200 dilution, Molecular Probes, Invitrogen).

Techniques: Transfection, Plasmid Preparation, Expressing, Incubation, Staining